People in Research

Mandana Veiseh

Advisor: Miqin Zhang

Department/status: Ph.D. Candidate

Project overview: Protein and cell patterning for cell-based biosensors

Project details:
The central component of the research is to develop novel techniques to pattern cells with high precision, selectivity, reproducibility, and long-term stability on different types of Bio-MEMS for cell-based biosensors. Main application: drug screening.

Skills:

• Bio-MEMS: Developed a highly selective protein and cell patterning technique for Bio-MEMS
• Gene Expression and Gene Delivery:
  - Cloned Green fluorescence protein to mammalian cells and applied them on bio-chips for drug screening
  - Delivered "Virus AAVlacz" to bio-chips containing patterned fibroblast cells for gene therapy
  
  
• Single cell analysis on bio-chips: Performed single cell analysis by synchrotron infrared spectromicroscopy on various types of adhered mammalian cells on bio-chips
  
  
• Biocompatibility: - Designed and conducted experiments for synthesis of polyethylene glycol (PEG) molecules with different functional groups and chain lengths
  - Performed biocompatibility tests for different types of PEG-treated surfaces
  
  
• Surface Engineering: Engineered BioMEMS surfaces (Au, Si, Au/Si, and Ti) by self-assembly, covalent and physical binding techniques
  
  
• Surface Characterization: Characterized bio-chips at key steps of surface engineering by ToF-SIMS, AFM, FTIR, ESCA, Ellipsometry, Fluorescence microscopy, contact angle and optical microscopy
  
  
• Material Science
  - Instron (tensile, compression and flexural tests)
  - Rockwell Hardness
  - Metal polishing
  - Cold working
  
  
• Textile Chemistry
  - Color matching of fluorescent samples by Neural Network
  - Textile Dyeing and Printing techniques
  - Textile fastness measurement techniques
  

Relevant honors or funding:
2004 Outstanding Female Recipient Award, Society of Women Engineers
2003 Advanced Light Source Doctoral Fellowship, Lawrence Berkeley National Lab
2003 College of Engineering L&K Marsh Fellowship, University of Washington

Names of others on the project
Undergraduates: Joyce Tseng (MSE Senior), Lisamarie Ramos (MSE Senior)
Research staff: Omid Veiseh